Phosphoramide mustard

CAS No. 10159-53-2

Phosphoramide mustard( —— )

Catalog No. M35039 CAS No. 10159-53-2

Phosphoramide mustard is a toxic metabolite of cyclophosphamide with anticancer activity and ovarian toxicity that induces DNA damage.

Purity : >98% (HPLC)

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Biological Information

  • Product Name
    Phosphoramide mustard
  • Note
    Research use only, not for human use.
  • Brief Description
    Phosphoramide mustard is a toxic metabolite of cyclophosphamide with anticancer activity and ovarian toxicity that induces DNA damage.
  • Description
    Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage.
  • In Vitro
    Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs).Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.Cell Viability Assay Cell Line:SIGCs Concentration:0.5 μM, 1 μM, 3 μM, 6 μM Incubation Time:48 hours Result:Reduced cell viability at concentrations of 3 μM and higher.RT-PCR Cell Line:SIGCs Concentration:3 μM, 6 μM Incubation Time:24 hours, 48 hours Result:Increased DDR gene mRNA expression levels. Western Blot Analysis Cell Line:SIGCs Concentration: 3 μM, 6 μM Incubation Time:24 hours, 48 hours Result:Generally increased DDR proteins.
  • In Vivo
    Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).Animal Model:Rat, subcutaneously implanted Walker 256 carcinosarcoma tumorDosage:2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg Administration:Intraperitoneal injection, once daily, for 5 consecutive days Result:Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.Animal Model:Rats Dosage:59.4 mg/kg (Pharmacokinetic Analysis)Administration:Intravenous injection Result:T1/2 (15.1 min).
  • Synonyms
    ——
  • Pathway
    Others
  • Target
    Other Targets
  • Recptor
    Drug Metabolite | DNA Alkylation
  • Research Area
    ——
  • Indication
    ——

Chemical Information

  • CAS Number
    10159-53-2
  • Formula Weight
    221.02
  • Molecular Formula
    C4H11Cl2N2O2P
  • Purity
    >98% (HPLC)
  • Solubility
    ——
  • SMILES
    O=P(O)(N)N(CCCl)CCCl
  • Chemical Name
    ——

Shipping & Storage Information

  • Storage
    (-20℃)
  • Shipping
    With Ice Pack
  • Stability
    ≥ 2 years

Reference

1. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258.?
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