Phosphoramide mustard

Research use only, not for human use.

Phosphoramide mustard is a toxic metabolite of cyclophosphamide with anticancer activity and ovarian toxicity that induces DNA damage.

Phosphoramide mustard is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard induces DNA damage.

Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.Phosphoramide mustard (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs).Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.Phosphoramide mustard (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.Cell Viability Assay Cell Line:SIGCs Concentration:0.5 μM, 1 μM, 3 μM, 6 μM Incubation Time:48 hours Result:Reduced cell viability at concentrations of 3 μM and higher.RT-PCR Cell Line:SIGCs Concentration:3 μM, 6 μM Incubation Time:24 hours, 48 hours Result:Increased DDR gene mRNA expression levels. Western Blot Analysis Cell Line:SIGCs Concentration: 3 μM, 6 μM Incubation Time:24 hours, 48 hours Result:Generally increased DDR proteins.

Phosphoramide mustard (2.1-20.7 mg/kg; i.p.; daily; for 5 days) inhibits subcutaneous tumor growth in rats.Phosphoramide mustard exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg).Animal Model:Rat, subcutaneously implanted Walker 256 carcinosarcoma tumorDosage:2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg Administration:Intraperitoneal injection, once daily, for 5 consecutive days Result:Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.Animal Model:Rats Dosage:59.4 mg/kg (Pharmacokinetic Analysis)Administration:Intravenous injection Result:T1/2 (15.1 min).

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Others

Other Targets

Drug Metabolite | DNA Alkylation

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10159-53-2

221.02

C4H11Cl2N2O2P

>98% (HPLC)

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O=P(O)(N)N(CCCl)CCCl

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(-20℃)

With Ice Pack

≥ 2 years